Glutamate ELISA 试剂盒
适用: 化学剂
Competition ELISA
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- 抗原 See all Glutamate products
- Glutamate
- 适用
- 化学剂
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of Glutamate in the research laboratory
Alternative Names: Glutamate ELISA kit
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Glutamate Assay Kit
BCA Food, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal conditions to be determined by end user
- 实验流程
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After extraction and derivatization Glutamate is determined by ELISA methodology. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. Thereaction is monitored at 450 nm.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all Glutamate products
- Glutamate
- Abstract
- Glutamate 产品
- 物质类
- Amino Acid
- 背景
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Glutamic acid (abbreviated as Glu or E) is one of the 20 proteinogenic amino acids, and its codons are GAA and GAG. It is a nonessential amino acid. The carboxylate anions and salts of glutamic acid are known as glutamates. In neuroscience, glutamate is an important neurotransmitter that plays a key role in long-term potentiation and is important for learning and memory. Glutamate is a key molecule in cellular metabolism. In humans, dietary proteins are broken down by digestion into amino acids, which serve as metabolic fuel for other functional roles in the body. A key process in amino acid degradation is transamination, in which the amino group of an amino acid is transferred to an alpha-ketoacid, typically catalysed by a transaminase.
Synonyms: Glutamate ELISA kit.
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