Histamine ELISA 试剂盒
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- 抗原 See all Histamine (HIS) ELISA试剂盒
- Histamine (HIS)
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适用
- 化学剂
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of Histamine in foodstuffs in the research laboratory
Alternative Names: Histamine ELISA kit - Top Product
- Discover our top product HIS ELISA Kit
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Histamine (HIS) ELISA Kit
HIS 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 人 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 兔 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Histamine (HIS) ELISA KitHIS 适用: Various Species Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal conditions to be determined by end-user
- 实验流程
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The assay kit provides materials for the quantitative determination of derivatized histamine in food extracts. The derivatization is part of the preparation of the samples. By using the acylation reagent, histamine is derivatized into Nacylhistamine. The competitive Histamine EIA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by antigoat/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all Histamine (HIS) ELISA试剂盒
- Histamine (HIS)
- 别名
- Histamine (HIS 产品)
- 物质类
- Chemical
- 背景
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Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Histamine ELISA kit.
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