DENV IgM ELISA 试剂盒
DENV IgM
适用: Dengue Virus
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- 抗原 See all DENV IgM products
- DENV IgM (Dengue Virus Antibody IgM (DENV IgM))
- 适用
- Dengue Virus
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of Dengue Virus IgM in the research laboratory
Alternative Names: Dengue Virus IgM ELISA kit
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- 应用备注
- Optimal conditions to be determined by end user
- 实验流程
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Diluted patient serum (serum diluent contains sorbent to remove rheumatoid factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibodyantigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all DENV IgM products
- DENV IgM (Dengue Virus Antibody IgM (DENV IgM))
- Abstract
- DENV IgM 产品
- 物质类
- Antibody
- 背景
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The mosquitoborne dengue viruses (serotype 14) cause dengue fever, a severe flulike illness. The disease is prevalent in Third World tropical regions and spreading to subtropical developed countries including the United States. WHO estimates that 5080 million cases of dengue fever occur worldwide each year, including a potentially deadly form of the disease called dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Primary infection with dengue virus results in a selflimiting disease characterized by mild to high fever lasting 3 to 7 days, severe headache with pain behind the eyes, muscle and joint pain, rash and vomiting. Secondary infection is the more common form of the disease in many parts of Southeast Asia and South America. This form of the disease is more serious and can result in DHF and DSS. The major clinical symptoms can include high fever, hemorrhagic events, and circulatory failure, and the fatality rate can be as high as 40 %. Early diagnosis of DSS is particularly important, as patients may die within 12 to 24 h if appropriate treatment is not administered. Primary dengue virus infection is characterized by elevations in specific IgM antibody levels 3 to 5 days after the onset of symptoms, this generally persists for 30 to 60 days. IgG levels also become elevated after 10 to 14 days and remain detectable for life. During secondary infection, IgM levels generally rise more slowly and reach lower levels than in primary infection, while IgG levels rise rapidly from 1 to 2 days after the onset of symptoms.
Synonyms: Dengue Virus IgM ELISA kit.
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