beta Catenin ELISA 试剂盒
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- 抗原 See all beta Catenin (CATNB) ELISA试剂盒
- beta Catenin (CATNB) (Catenin, beta (CATNB))
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适用
- 犬
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of beta Catenin in the research laboratory
Alternative Names: beta Catenin ELISA kit, CTNNB ELISA kit, - Top Product
- Discover our top product CATNB ELISA Kit
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Catenin, beta (CATNB) ELISA Kit
CATNB 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Catenin, beta (CATNB) ELISA KitCATNB 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal conditions to be determined by end user
- 实验流程
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This betacatenin ELISA kit is a complete kit for the determination of betacatenin in cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to betacatenin immobilized on a microtiter plate to bind the betacatenin in the standards or sample. A recombinant human betacatenin Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to betacatenin is added. This antibody binds to the betacatenin captured on the plate. After a short incubation the excess antibody is washed out and Goat antiRabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal betacatenin antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of betacatenin in either standards or samples.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all beta Catenin (CATNB) ELISA试剂盒
- beta Catenin (CATNB) (Catenin, beta (CATNB))
- 别名
- beta Catenin (CATNB 产品)
- 背景
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Beta Catenin is a 781 amino acid adherens junction protein that plays an important role in two unrelated cellular functions. Adherens junctions are critical for establishment and maintenance of epithelial layers, such as those found lining the surface of organs. Involved in both cell-cell adhesion and in the wnt/wg signaling pathway, beta Catenin can be found in the cytoplasm or the nucleus. In the cytoplasm, beta Catenin is unstabilized by phosphorylation and association with E-cadherin. When beta Catenin translocates to the nucleus, it has comparatively low levels of phosphorylation. Wnt/wg signaling results in beta Catenin accumulation and transcriptional activation of specific target genes during development. Deregulation of beta Catenin signaling is an important event in the evolution a number of malignancies, including colon cancer, melanoma, ovarian cancer, and prostate cancer. The homology between human, mouse and rat betacatenin is 99.9 %.
Synonyms: Catenin beta ELISA kit, CTNNB ELISA kit,. - 途径
- Peptide Hormone Metabolism
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