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Calcitonin ELISA 试剂盒

Calca 适用: 人 Colorimetric Sandwich ELISA
产品编号 ABIN930354
发货至: 中国
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    Calcitonin (Calca)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    Analytical Method
    Quantitative
    产品特性
    ELISA kit for the detection of Calcitonin in the research laboratory
    Alternative Names: Calcitonin ELISA kit, CT ELISA kit
    Top Product
    Discover our top product Calca ELISA Kit
  • 应用备注
    Optimal conditions to be determined by end user
    板类型
    Pre-coated
    实验流程

    The Calcitonin Immunoassay is a twosite ELISA [EnzymeLinked ImmunoSorbent Assay] for the measurement of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for welldefined regions on the calcitonin molecule. One antibody binds only to Calcitonin 1123 and this antibody is biotinylated. The other antibody binds only to Calcitonin 2132 and this antibody is labeled withhorseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidincoated microplate well.Thus the calcitonin in the sample is sandwiched between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in thesample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and patient samples are determined directly from this curve.

    限制
    仅限研究用
  • 储存条件
    4 °C
    储存方法
    Store at 2-8 °C.
  • 抗原 See all Calcitonin (Calca) ELISA试剂盒
    Calcitonin (Calca)
    别名
    Calcitonin (Calca 产品)
    背景
    Calcitonin (CT) is a 32 amino acid peptide hormone secreted by the parafollicular C cells of the thyroid gland under serum calcium control. After acute administration, this peptide acts as a potent hypocalcemic and hypophosphatemic hormone by increasing renal calcium clearance and reducing bone resorption. However, its precise physiological role in bone metabolism is not yet fully understood. Various forms of CT may be detected in blood samples, including a CT monomer, an oxidized monomer, a dimer, higher molecular weight forms, and possibly precursor of CT. The concentrations of these peptides vary with clinical status, renal function and tissular origin of CT (normal or ectopic production). Medullary thyroid carcinoma (MTC) is a malignant tumor, developed from the C cells, secreting calcitonin in large excess. This disease occurs either as a sporadic (80 %) or a familial (20 %) form, which is transmitted as an autosomal dominant gene or as a component of multiple endocrine neoplasia (IIb). Moderate hypercalcitoninemia is also observed in pregnancy, pernicious anaemia, renal failure and during the neonatal period. Preferably, monomer form of CT is detected in this assay.
    Synonyms: Calcitonin ELISA kit, CT ELISA kit.
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