CEACAM5 ELISA 试剂盒
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- 抗原 See all CEACAM5 ELISA试剂盒
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
- 抗原表位
- AA 35-685
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CEA
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: K35-A685 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: K35-A685 - Featured
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Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) ELISA Kit
CEACAM5 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) ELISA KitCEACAM5 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the immunoglobulin superfamily. CEA family.
Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
- 板类型
- Pre-coated
- 实验流程
- human CEA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEA has been precoated onto 96-well plates. Standards(NSO, K35-A685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEA amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CEA standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.36, Standard deviation: 0.113, CV(%): 4.8
- Sample 2: n=16, Mean(ng/ml): 5.59, Standard deviation: 0.235, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 10.4, Standard deviation: 0.53, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.171, CV(%): 6.9
- Sample 2: n=24, Mean(ng/ml): 5.56, Standard deviation: 0.395, CV(%): 7.1
- Sample 3: n=24, Mean(ng/ml): 10.7, Standard deviation: 0.556, CV(%): 5.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Ultrasensitive all-carbon photoelectrochemical bioprobes for zeptomole immunosensing of tumor markers by an inexpensive visible laser light." in: Analytical chemistry, Vol. 85, Issue 21, pp. 10612-9, (2013) (PubMed).
-
: "Ultrasensitive all-carbon photoelectrochemical bioprobes for zeptomole immunosensing of tumor markers by an inexpensive visible laser light." in: Analytical chemistry, Vol. 85, Issue 21, pp. 10612-9, (2013) (PubMed).
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- 抗原 See all CEACAM5 ELISA试剂盒
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
- 别名
- CEACAM5 (CEACAM5 产品)
- 别名
- CD66e ELISA Kit, CEA ELISA Kit, CEACAM5 ELISA Kit, CEACAM ELISA Kit, 1600029H12Rik ELISA Kit, Psg30 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 8 ELISA Kit, carcinoembryonic antigen gene family ELISA Kit, CEACAM5 ELISA Kit, Ceacam5 ELISA Kit, LOC100409241 ELISA Kit, Cea ELISA Kit
- 背景
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Protein Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .
Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60 % carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Synonyms: Carcinoembryonic antigen-related cell adhesion molecule 5,Carcinoembryonic antigen,CEA,Meconium antigen 100,CD66e,CEACAM5,CEA,
Full Gene Name: Carcinoembryonic antigen-related cell adhesion molecule 5
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. - 基因ID
- 111518
- UniProt
- P06731
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