HAVCR1 ELISA 试剂盒
(3 references)-
- 抗原 See all HAVCR1 ELISA试剂盒
- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
- 抗原表位
- AA 21-288
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KIM1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: S21-T288 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <2pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: S21-T288 - Featured
- Discover our best selling HAVCR1 ELISA Kit
- Top Product
- Discover our top product HAVCR1 ELISA Kit
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Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
HAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Plasma, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the immunoglobulin superfamily. TIM family.
Tissue Specificity: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. .
- 板类型
- Pre-coated
- 实验流程
- human KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S21-T288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KIM1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KIM1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.64, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 746, Standard deviation: 38.8, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 1220, Standard deviation: 74.42, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 317, Standard deviation: 20.3, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 882, Standard deviation: 59.1, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 1458, Standard deviation: 115.2, CV(%): 7.9
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis." in: Scientific reports, Vol. 8, Issue 1, pp. 7962, (2018) (PubMed).
: "Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP and CYS C levels in urine." in: International braz j urol : official journal of the Brazilian Society of Urology, Vol. 43, Issue 5, pp. 887-895, (2017) (PubMed).
: "Renoprotective effects of berberine as adjuvant therapy for hypertensive patients with type 2 diabetes mellitus: Evaluation via biochemical markers and color Doppler ultrasonography." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 869-876, (2015) (PubMed).
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: "Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis." in: Scientific reports, Vol. 8, Issue 1, pp. 7962, (2018) (PubMed).
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- 抗原 See all HAVCR1 ELISA试剂盒
- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
- 别名
- HAVCR1 (HAVCR1 产品)
- 别名
- HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit
- 物质类
- Virus
- 背景
-
Protein Function: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. .
Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.
Synonyms: Hepatitis A virus cellular receptor 1,HAVcr-1,Kidney injury molecule 1,KIM-1,T-cell immunoglobulin and mucin domain-containing protein 1,TIMD-1,T-cell immunoglobulin mucin receptor 1,TIM,TIM-1,T-cell membrane protein 1,HAVCR1,KIM1, TIM1, TIMD1,
Full Gene Name: Hepatitis A virus cellular receptor 1
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 26762
- UniProt
- Q96D42
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