Fetuin A ELISA 试剂盒
(3 references)-
- 抗原 See all Fetuin A (AHSG) ELISA试剂盒
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
- 抗原表位
- AA 19-367
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fetuin A
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: A19-V367 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: A19-V367 - Featured
- Discover our best selling AHSG ELISA Kit
- Top Product
- Discover our top product AHSG ELISA Kit
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alpha-2-HS-Glycoprotein (AHSG) ELISA Kit
AHSG 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: Cow Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 猴 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: 兔 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: Pig Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-HS-Glycoprotein (AHSG) ELISA KitAHSG 适用: Cow Colorimetric Competition ELISA 125-8000 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the fetuin family.
Tissue Specificity: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
- 板类型
- Pre-coated
- 实验流程
- human Fetuin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fetuin A has been precoated onto 96-well plates. Standards(NSO, A19-V367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fetuin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fetuin A amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Fetuin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Fetuin A standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 2.17, Standard deviation: 0.089, CV(%): 4.1
- Sample 2: n=16, Mean(ng/ml): 8.32, Standard deviation: 0.441, CV(%): 5.3
- Sample 3: n=16, Mean(ng/ml): 12.55, Standard deviation: 0.602, CV(%): 4.8,
- Sample 1: n=24, Mean(ng/ml): 2.39, Standard deviation: 0.129, CV(%): 5.4
- Sample 2: n=24, Mean(ng/ml): 8.75, Standard deviation: 0.543, CV(%): 6.2
- Sample 3: n=24, Mean(ng/ml): 13.64, Standard deviation: 0.777, CV(%): 5.7
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).
: "Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes." in: BMC immunology, Vol. 17, Issue 1, pp. 33, (2016) (PubMed).
: "Association Between Cardiac Valvular Calcification and Serum Fetuin-A Levels in Renal Transplant Recipients." in: Transplantation proceedings, Vol. 47, Issue 5, pp. 1398-401, (2015) (PubMed).
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: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).
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- 抗原 See all Fetuin A (AHSG) ELISA试剂盒
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
- 别名
- AHSG (AHSG 产品)
- 别名
- ahs ELISA Kit, a2hs ELISA Kit, hsga ELISA Kit, fetua ELISA Kit, wu:fb63g02 ELISA Kit, zgc:103687 ELISA Kit, AHSG ELISA Kit, MGC116429 ELISA Kit, A2HS ELISA Kit, AHS ELISA Kit, FETUA ELISA Kit, HSGA ELISA Kit, Aa2-066 ELISA Kit, pp63 ELISA Kit, alpha-2-HS-glycoprotein ELISA Kit, alpha 2-HS glycoprotein ELISA Kit, alpha-2-HS-glycoprotein 1 ELISA Kit, alpha-2-HS-glycoprotein L homeolog ELISA Kit, Cphamn1_1981 ELISA Kit, AHSG ELISA Kit, ahsg ELISA Kit, ahsg1 ELISA Kit, ahsg.L ELISA Kit, Ahsg ELISA Kit
- 背景
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Protein Function: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Background: Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2
Synonyms: Alpha-2-HS-glycoprotein,Alpha-2-Z-globulin,Ba-alpha-2-glycoprotein,Fetuin-A,Alpha-2-HS-glycoprotein chain A,Alpha-2-HS-glycoprotein chain B,AHSG,FETUA,PRO2743,
Full Gene Name: Alpha-2-HS-glycoprotein
Cellular Localisation: Secreted. - 基因ID
- 197
- UniProt
- P02765
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