Cathepsin D ELISA 试剂盒
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- 抗原 See all Cathepsin D (CTSD) ELISA试剂盒
- Cathepsin D (CTSD)
- 抗原表位
- AA 21-412
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin D
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: L21-L412 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: L21-L412 - Featured
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Cathepsin D (CTSD) ELISA Kit
CTSD 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitHigh Sensitivity CTSD 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD 适用: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
SensoLyte® 390 Cathepsin D Assay KitFRET Fluorometric
SensoLyte® 520 Cathepsin D Assay KitFRET Fluorometric
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase A1 family.
Tissue Specificity: Expressed in the aorta extrcellular space (at protein level). .
- 板类型
- Pre-coated
- 实验流程
- human Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, L21-L412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin D amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin D standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.24, Standard deviation: 0.035, CV(%): 2.8
- Sample 2: n=16, Mean(ng/ml): 3.37, Standard deviation: 0.108, CV(%): 3.2
- Sample 3: n=16, Mean(ng/ml): 6.73, Standard deviation: 0.236, CV(%): 3.5,
- Sample 1: n=24, Mean(ng/ml): 1.38, Standard deviation: 0.047, CV(%): 3.4
- Sample 2: n=24, Mean(ng/ml): 3.51, Standard deviation: 0.130, CV(%): 3.7
- Sample 3: n=24, Mean(ng/ml): 6.82, Standard deviation: 0.28, CV(%): 4.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes." in: Disease markers, Vol. 2017, pp. 5286408, (2018) (PubMed).
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: "Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes." in: Disease markers, Vol. 2017, pp. 5286408, (2018) (PubMed).
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- 抗原 See all Cathepsin D (CTSD) ELISA试剂盒
- Cathepsin D (CTSD)
- 别名
- CTSD (CTSD 产品)
- 别名
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- 背景
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Protein Function: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Synonyms: Cathepsin D,3.4.23.5,Cathepsin D light chain,Cathepsin D heavy chain,CTSD,CPSD,
Full Gene Name: Cathepsin D
Cellular Localisation: Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).. - 基因ID
- 1509
- UniProt
- P07339
- 途径
- Peptide Hormone Metabolism
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