L-Selectin ELISA 试剂盒
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- 抗原 See all L-Selectin (SELL) ELISA试剂盒
- L-Selectin (SELL) (Selectin L (SELL))
- 抗原表位
- AA 39-332
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适用
- 化学剂
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sL-Selectin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: W39-N332 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <5pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: W39-N332 - Featured
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Selectin L (SELL) ELISA Kit
SELL 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Selectin L (SELL) ELISA KitSELL 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Selectin L (SELL) ELISA KitSELL 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin L (SELL) ELISA KitSELL 适用: 化学剂 Colorimetric Sandwich ELISA 35-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Selectin L (SELL) ELISA KitSELL 适用: 化学剂 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Selectin L (SELL) ELISA KitSELL 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Selectin L (SELL) ELISA KitSELL 适用: 猴 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Selectin L (SELL) ELISA KitSELL 适用: 化学剂 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Selectin L (SELL) ELISA KitSELL 适用: 化学剂 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma, Serum
Selectin L (SELL) ELISA KitSELL 适用: 化学剂 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the selectin/LECAM family.
Tissue Specificity: Expressed in B-cell lines and T-lymphocytes. .
- 板类型
- Pre-coated
- 实验流程
- human sL-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sL-Selectin has been precoated onto 96-well plates. Standards(NSO, W39-N332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sL-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sL-Selectin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human sL-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human sL-Selectin standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 679, Standard deviation: 36, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 1358, Standard deviation: 53, CV(%): 3.9
- Sample 3: n=16, Mean(pg/ml): 2127, Standard deviation: 110.6, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 799, Standard deviation: 44.7, CV(%): 5.6
- Sample 2: n=24, Mean(pg/ml): 1643, Standard deviation: 79.1, CV(%): 4.8
- Sample 3: n=24, Mean(pg/ml): 2585, Standard deviation: 191.3, CV(%): 7.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes." in: Journal of virology, Vol. 89, Issue 10, pp. 5687-700, (2015) (PubMed).
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: "HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes." in: Journal of virology, Vol. 89, Issue 10, pp. 5687-700, (2015) (PubMed).
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- 抗原 See all L-Selectin (SELL) ELISA试剂盒
- L-Selectin (SELL) (Selectin L (SELL))
- 别名
- SELL (SELL 产品)
- 别名
- CD62L ELISA Kit, LAM1 ELISA Kit, LECAM1 ELISA Kit, LEU8 ELISA Kit, LNHR ELISA Kit, LSEL ELISA Kit, LYAM1 ELISA Kit, PLNHR ELISA Kit, TQ1 ELISA Kit, A.11 ELISA Kit, L-selectin ELISA Kit, LECAM-1 ELISA Kit, SELE ELISA Kit, AI528707 ELISA Kit, Lnhr ELISA Kit, Ly-22 ELISA Kit, Ly-m22 ELISA Kit, Lyam-1 ELISA Kit, Lyam1 ELISA Kit, selectin L ELISA Kit, selectin, lymphocyte ELISA Kit, SELL ELISA Kit, Sell ELISA Kit
- 背景
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Protein Function: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .
Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Synonyms: L-selectin,CD62 antigen-like family member L,Leukocyte adhesion molecule 1,LAM-1,Leukocyte surface antigen Leu-8,Leukocyte-endothelial cell adhesion molecule 1,LECAM1,Lymph node homing receptor,TQ1,gp90-MEL,CD62L,SELL,LNHR, LYAM1,
Full Gene Name: L-selectin
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 6402
- UniProt
- P14151
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