CCL5 ELISA 试剂盒
(3 references)-
- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- 抗原表位
- AA 24-91
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Rantes
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S24-S91 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S24-S91 - Featured
- Discover our best selling CCL5 ELISA Kit
- Top Product
- Discover our top product CCL5 ELISA Kit
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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: T-cell and macrophage specific.
- 板类型
- Pre-coated
- 实验流程
- mouse Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Rantes has been precoated onto 96-well plates. Standards(E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Rantes amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Rantes standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Rantes standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 127, Standard deviation: 6.73, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 285, Standard deviation: 18.24, CV(%): 6.4
- Sample 3: n=16, Mean(pg/ml): 498, Standard deviation: 35.9, CV(%): 7.2,
- Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 12.12, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 312, Standard deviation: 21.22, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 587, Standard deviation: 47.55, CV(%): 8.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580." in: International journal of chronic obstructive pulmonary disease, Vol. 11, pp. 909-17, (2017) (PubMed).
: "Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 209, (2017) (PubMed).
: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).
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: "In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580." in: International journal of chronic obstructive pulmonary disease, Vol. 11, pp. 909-17, (2017) (PubMed).
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- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- 别名
- CCL5 (CCL5 产品)
- 别名
- Rantes ELISA Kit, Scya5 ELISA Kit, D17S136E ELISA Kit, RANTES ELISA Kit, SCYA5 ELISA Kit, SIS-delta ELISA Kit, SISd ELISA Kit, TCP228 ELISA Kit, eoCP ELISA Kit, MuRantes ELISA Kit, C-C motif chemokine 5-like ELISA Kit, C-C motif chemokine ligand 5 ELISA Kit, chemokine (C-C motif) ligand 5 ELISA Kit, LOC100230935 ELISA Kit, CCL5 ELISA Kit, Ccl5 ELISA Kit
- 背景
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Protein Function: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. .
Background: Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
Synonyms: C-C motif chemokine 5,MuRantes,SIS-delta,Small-inducible cytokine A5,T-cell-specific protein RANTES,Ccl5,Scya5,
Full Gene Name: C-C motif chemokine 5
Cellular Localisation: Secreted. - 基因ID
- 20304
- UniProt
- P30882
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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