MMP13 ELISA 试剂盒

Sequence similarities: Belongs to the peptidase M10A family.

Tissue Specificity: Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue. .

Aliquot 0.1 mL per well of 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP13 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP13 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 471, Standard deviation: 19.8, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1623, Standard deviation: 86.02, CV(%): 5.3
• Sample 3: n=16, Mean(pg/ml): 3245, Standard deviation: 194.7, CV(%): 6,
• Sample 1: n=24, Mean(pg/ml): 538, Standard deviation: 29.1, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 1776, Standard deviation: 104.8, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 3685, Standard deviation: 265.32, CV(%): 7.2

Protein Function: Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. .

Background: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Synonyms: Collagenase 3,3.4.24.-,Matrix metalloproteinase-13,MMP-13,MMP13,

Full Gene Name: Collagenase 3