CCL20 ELISA 试剂盒
(1 reference)-
- 抗原 See all CCL20 ELISA试剂盒
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
- 抗原表位
- AA 27-96
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8-500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MIP-3 alpha/CCL20
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S27-M96 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S27-M96 - Featured
- Discover our best selling CCL20 ELISA Kit
- Top Product
- Discover our top product CCL20 ELISA Kit
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Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA Kit
CCL20 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 人 AA 27-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 AA 28-97 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 3-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Low levels in thymus and lung.
- 板类型
- Pre-coated
- 实验流程
- rat MIP-3 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-3 alpha has been precoated onto 96-well plates. Standards(E.coli, S27-M96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-3 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-3 alpha amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MIP-3 alpha standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 48, Standard deviation: 2.45, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 145, Standard deviation: 6.67, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 273, Standard deviation: 17.2, CV(%): 6.3,
- Sample 1: n=24, Mean(pg/ml): 67, Standard deviation: 4.36, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 162, Standard deviation: 8.75, CV(%): 5.4
- Sample 3: n=24, Mean(pg/ml): 294, Standard deviation: 22.05, CV(%): 7.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "MicroRNA-132 enhances transition from inflammation to proliferation during wound healing." in: The Journal of clinical investigation, Vol. 125, Issue 8, pp. 3008-26, (2015) (PubMed).
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: "MicroRNA-132 enhances transition from inflammation to proliferation during wound healing." in: The Journal of clinical investigation, Vol. 125, Issue 8, pp. 3008-26, (2015) (PubMed).
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- 抗原 See all CCL20 ELISA试剂盒
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
- 别名
- CCL20 (CCL20 产品)
- 别名
- MIP-3ALPHA ELISA Kit, CCL20 ELISA Kit, si:dkey-150o13.1 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP-3-alpha ELISA Kit, MIP-3a ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, MIP-3A ELISA Kit, MIP-3[a] ELISA Kit, Scya20 ELISA Kit, exodus-1 ELISA Kit, C-C motif chemokine ligand 20 ELISA Kit, chemokine (C-C motif) ligand 20b ELISA Kit, chemokine (C-C motif) ligand 20 ELISA Kit, CCL20 ELISA Kit, ccl20b ELISA Kit, Ccl20 ELISA Kit
- 背景
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Protein Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (By similarity). May play a role in modulating inflammatory cell recruitment to the CNS and therefore contribute to tissue injury in ischemic stroke and autoimmune diseases. .
Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
Synonyms: C-C motif chemokine 20,Beta-chemokine exodus-1,CC chemokine LARC,CC chemokine ST38,Liver and activation-regulated chemokine,Macrophage inflammatory protein 3 alpha,MIP-3-alpha,Small-inducible cytokine A20,Ccl20,Scya20, St38,
Full Gene Name: C-C motif chemokine 20
Cellular Localisation: Secreted. - 基因ID
- 29538
- UniProt
- P97884
- 途径
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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