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IL-6 ELISA 试剂盒

Name: IL-6 ELISA 试剂盒
SKU: ABIN921064
Availability: OutOfStock

IL6 适用: 大鼠 AA 25-211 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

ELISA

1 image

(144 references)

产品编号 ABIN921064

发货至: 中国

Datasheet as PDF

抗原 See all IL-6 (IL6) ELISA试剂盒

IL-6 (IL6) (Interleukin 6 (IL6))

抗原表位

AA 25-211
适用
24

17

17

9

8

8

5

5

5

4

4

4

2

2

2

2

2

1

1

1
大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

最低检测浓度

62.5 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-6

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: F25-T211

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<5pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: F25-T211

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

rat IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-6 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-6 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 150, Standard deviation: 13.65, CV(%): 9.1
- Sample 2: n=16, Mean(pg/ml): 392, Standard deviation: 34.89, CV(%): 8.9
- Sample 3: n=16, Mean(pg/ml): 1635, Standard deviation: 91.56, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 176, Standard deviation: 16.72, CV(%): 9.5
- Sample 2: n=24, Mean(pg/ml): 415, Standard deviation: 38.18, CV(%): 9.2
- Sample 3: n=24, Mean(pg/ml): 1474, Standard deviation: 110.55, CV(%): 7.5
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
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抗原 See all IL-6 (IL6) ELISA试剂盒

IL-6 (IL6) (Interleukin 6 (IL6))

别名

IL6 (IL6 产品)

别名

BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, IL-6 ELISA Kit, Il-6 ELISA Kit, ILg6 ELISA Kit, Ifnb2 ELISA Kit, il6 ELISA Kit, CHIL-6 ELISA Kit, interleukin 6 ELISA Kit, interleukin-6 ELISA Kit, IL6 ELISA Kit, Il6 ELISA Kit, il-6 ELISA Kit, IL-6 ELISA Kit

背景

Protein Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity). .
Background: Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.
Synonyms: Interleukin-6,IL-6,Il6,Il-6,
Full Gene Name: Interleukin-6
Cellular Localisation: Secreted.

基因ID

24498

UniProt

P20607

途径

TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome