BMP5 ELISA 试剂盒
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- 抗原 See all BMP5 ELISA试剂盒
- BMP5 (Bone Morphogenetic Protein 5 (BMP5))
- 抗原表位
- AA 317-454
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-5
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Osseous Tissue
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A317-H454 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 预测反应
- Bovine,Canine,Horse,Monkey,Rabbit
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A317-H454 - Featured
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- Top Product
- Discover our top product BMP5 ELISA Kit
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Bone Morphogenetic Protein 5 (BMP5) ELISA Kit
BMP5 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 人 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 马 AA 317-454 Colorimetric Sandwich ELISA
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Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 猴 AA 317-454 Colorimetric Sandwich ELISA
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Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 犬 AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: Cow AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 人 Colorimetric Sandwich ELISA 0.25-60 ng/mL Cell Culture Supernatant, Plasma, Serum
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Bone, Cell Culture Supernatant, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the TGF-beta family.
Tissue Specificity: Expressed in the lung and liver.
- 板类型
- Pre-coated
- 实验流程
- human BMP-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-5 has been precoated onto 96-well plates. Standards(NSO, A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-5 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human BMP-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or bone tissue to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-5 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.059, CV(%): 4.8
- Sample 2: n=16, Mean(ng/ml): 3.46, Standard deviation: 0.180, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 6.92, Standard deviation: 0.388, CV(%): 5.6,
- Sample 1: n=24, Mean(ng/ml): 1.38, Standard deviation: 0.073, CV(%): 5.3
- Sample 2: n=24, Mean(ng/ml): 3.57, Standard deviation: 0.203, CV(%): 5.7
- Sample 3: n=24, Mean(ng/ml): 6.85, Standard deviation: 0.425, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all BMP5 ELISA试剂盒
- BMP5 (Bone Morphogenetic Protein 5 (BMP5))
- 别名
- BMP5 (BMP5 产品)
- 别名
- bmp5l ELISA Kit, hm:zehl0669 ELISA Kit, zehl0669 ELISA Kit, zgc:64230 ELISA Kit, BMP5 ELISA Kit, AU023399 ELISA Kit, se ELISA Kit, bone morphogenetic protein 5 ELISA Kit, bmp5 ELISA Kit, BMP5 ELISA Kit, LOC100539495 ELISA Kit, Bmp5 ELISA Kit
- 背景
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Protein Function: Induces cartilage and bone formation.
Background: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
Synonyms: Bone morphogenetic protein 5,BMP-5,BMP5,
Full Gene Name: Bone morphogenetic protein 5
Cellular Localisation: Secreted. - 基因ID
- 653
- UniProt
- P22003
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
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