ANG ELISA 试剂盒
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- 抗原 See all ANG ELISA试剂盒
- ANG (Angiogenin (ANG))
- 抗原表位
- AA 25-147
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANG
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: Q25-P147 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <12pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: Q25-P147 - Featured
- Discover our best selling ANG ELISA Kit
- Top Product
- Discover our top product ANG ELISA Kit
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Angiogenin (ANG) ELISA Kit
ANG 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Angiogenin (ANG) ELISA KitANG 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Competition ELISA 94 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Angiogenin (ANG) ELISA KitANG 适用: Pig Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiogenin (ANG) ELISA KitANG 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the pancreatic ribonuclease family.
Tissue Specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .
- 板类型
- Pre-coated
- 实验流程
- human ANG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANG has been precoated onto 96-well plates. Standards(E.coli, Q25-P147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANG amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human ANG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANG standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 312, Standard deviation: 9.4, CV(%): 3
- Sample 2: n=16, Mean(pg/ml): 672, Standard deviation: 23.52, CV(%): 3.5
- Sample 3: n=16, Mean(pg/ml): 1663, Standard deviation: 54.9, CV(%): 3.3,
- Sample 1: n=24, Mean(pg/ml): 635, Standard deviation: 45.72, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 165.2, CV(%): 8.9
- Sample 3: n=24, Mean(pg/ml): 3489, Standard deviation: 300, CV(%): 8.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts." in: Investigative ophthalmology & visual science, Vol. 56, Issue 2, pp. 855-64, (2015) (PubMed).
: "Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes." in: PLoS ONE, Vol. 9, Issue 4, pp. e96117, (2014) (PubMed).
: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).
: "An isolated reactive synovitis of the sternoclavicular joint." in: Acta orthopaedica Belgica, Vol. 55, Issue 2, pp. 222-4, (1989) (PubMed).
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: "Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts." in: Investigative ophthalmology & visual science, Vol. 56, Issue 2, pp. 855-64, (2015) (PubMed).
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- 抗原 See all ANG ELISA试剂盒
- ANG (Angiogenin (ANG))
- 别名
- ANG (ANG 产品)
- 别名
- ALS9 ELISA Kit, HEL168 ELISA Kit, RNASE4 ELISA Kit, RNASE5 ELISA Kit, ANG ELISA Kit, AI385586 ELISA Kit, Ang1 ELISA Kit, Rnase5 ELISA Kit, Rnase5a ELISA Kit, ANG1 ELISA Kit, angiogenin ELISA Kit, angiogenin, ribonuclease, RNase A family, 5 ELISA Kit, ribonuclease A family member k6 ELISA Kit, ANG ELISA Kit, Ang ELISA Kit, RNASE6 ELISA Kit
- 背景
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Protein Function: Binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .
Background: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
Synonyms: Angiogenin,3.1.27.-,Ribonuclease 5,RNase 5,ANG,RNASE5,
Full Gene Name: Angiogenin
Cellular Localisation: Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA. - 基因ID
- 283
- UniProt
- P03950
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