HCP ELISA 试剂盒
HCP
适用: Chinese Hamster
Colorimetric
Sandwich ELISA
6.25 ng/mL - 400 ng/mL
Cell Culture Supernatant
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- 抗原 See all HCP products
- HCP (Host Cell Proteins (HCP))
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适用
- Chinese Hamster
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 ng/mL - 400 ng/mL
- 最低检测浓度
- 6.25 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.
- 样品类型
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- 交叉反应 （详细）
- No cross-reaction exists between the homologous antigens.
- 灵敏度
- 3.1 ng/mL
- 产品特性
- This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
- 组件
- Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 µL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验精密度
- Intra Variation%: 6-10, Inter Variation%: 6.9-10.4
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all HCP products
- HCP (Host Cell Proteins (HCP))
- 别名
- Host Cell Proteins (HCP 产品)
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