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HCP ELISA 试剂盒

HCP 适用: Chinese Hamster Colorimetric Sandwich ELISA 3.125 ng/mL – 100 ng/mL Cell Culture Supernatant
产品编号 ABIN7538037
发货至: 中国
  • 抗原 See all HCP products
    HCP (Host Cell Proteins (HCP))
    适用
    • 1
    • 1
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    • 1
    Chinese Hamster
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.125 ng/mL – 100 ng/mL
    最低检测浓度
    3.125 ng/mL
    应用范围
    ELISA
    原理
    The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.
    样品类型
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    交叉反应 (详细)
    No cross-reaction exists between the homologous antigens.
    灵敏度
    0.2 ng/mL
    产品特性
    This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
    组件
    Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    样本量
    100 µL
    实验时间
    3 h
    板类型
    Pre-coated
    实验精密度
    Intra Variation%: 3.1-4.5, Inter Variation%: 6.0-9.1
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all HCP products
    HCP (Host Cell Proteins (HCP))
    别名
    Host Cell Proteins (HCP 产品)
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