SARS-CoV-2 Spike ELISA 试剂盒
适用: SARS CoV-2 Omicron, SARS Coronavirus-2 (SARS-CoV-2)
RBD
Colorimetric
Sandwich ELISA
1.56 - 50 ng/mL
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- 抗原 See all SARS-CoV-2 Spike products
- SARS-CoV-2 Spike
- 抗原表位
- RBD
- 适用
- SARS CoV-2 Omicron, SARS Coronavirus-2 (SARS-CoV-2)
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 - 50 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- This kit is developed for detecting SARS-CoV-2 Spike RBD (XBB.1.5) in the sample, simultaneously can detect Omicron (BA.2 and BA.4 & BA.5 and BQ.1.1)
- Analytical Method
- Quantitative
- 特异性
- This kit is developed for detecting SARS-CoV-2 Spike RBD (XBB.1.5) in the sample, simultaneously can detect Omicron (BA.2 and BA.4 & BA.5 and BQ.1.1).
- 产品特性
- This assay kit is used to measure the levels of SARS-CoV-2 Spike RBD (XBB.1.5) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody. First add the standard samples provided in kit and your samples to the plate, incubate and wash the wells. Then add the Biotin-Anti-SARS-CoV-2 Spike RBD Antibody to the plate, incubate and wash the wells. Next add Streptavidin-HRP to the plate, incubate and wash the wells. Lastly load the substrate into the wells and monitor color development in proportion with the amount of Spike RBD (XBB.1.5) present. The reaction is stopped by the additionof a stop solution and the intensity of the absorbance can be measured at 450 nm and 630 nm. The OD Value reflects the amount of Spike RBD bound.
- 组件
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- Pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate
- SARS-CoV-2 Spike RBD (XBB.1.5)
- Biotin-Anti-SARS-CoV-2 Spike RBD Antibody
- Streptavidin-HRP
- 10 x Washing Buffer
- Dilution Buffer
- Substrate Solution
- Stop Solution
- 试剂未包括
- Single or dual wavelength microplate reader with 450 nm and 630 nm filter, Centrifuge, 37ºC Incubator, 10 µL, 200 µL and 1000 µL precision pipettes, 10 µL, 200 µL and 1000 µL pipette tips, Multichannel pipettes, Tubes, Graduated cylinder to prepare Wash Solution, Deionized or distilled water to dilute 10× Washing Buffer
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- 板类型
- Pre-coated
- 实验流程
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- Bring all reagents and samples to room temperature (20℃-25℃) before use.
- Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.
- Add the Secondary antibody biotin-Anti-SARS-CoV-2 Spike RBD Antibody diluted by Dilution Buffer to the plate.
- Add the diluted Streptavidin-HRP to the plate.
- Wash the plate and add TMB or other colorimetric HRP substrate.
- Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
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The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening. - 有效期
- 6 months
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- 抗原 See all SARS-CoV-2 Spike products
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike 产品
- 背景
- The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike RBD is urgently needed to accelerate the development of COVID-19 vaccines.
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