plexWell™ 96
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- 应用范围
- Library Preparation (LibPrep)
- 原理
- plexWell™96 Library Preparation Kit for Illumina® Sequencing Platforms
- 特异性
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- Kit contains major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
- Efficient library prep for one 96-well plate inputs
- Normalizes input DNA over wide input range of 3-30 ng
- Volume-based pricing (save 30-50 % on total lab costs)
- 产品特性
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plexWell NGS multiplexed library generation kit for Illumina®
- Assay-ready 96-well fully-skirted low-profile PCR plate
- 2,304 (96 x 24) unique barcode combinations
- 组件
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Box 1:
- Sample Barcode Plate (-20°C)
- 3X Coding Buffer (ambient)
- X Solution (ambient)
- MAGwise™ Paramagnetic Beads (4°C)
- Pool Barcode Reagent (-20°C)
- Library Primer Mix (-20°C)
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
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- Large scale full length viral surveillance
- Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
- Microbiome screening
- Microbial whole genome sequencing
- scRNA-seq
- Low-depth whole genome/GBS
- 说明
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Time-to-prepare a multiplexed library from 96 samples:
- Hands-on time: 50 min
- Total elapsed time: 3 hrs
Expected Results:- Library Yield: 750 - 1500 fmoles of purified multiplexed library
- Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
- Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
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Box 1 and Box 3 + cDNA: ship on dry ice, store at -20 °C
Box 2: ship ambient. Do NOT freeze!, store Magwise at 4 °C and Coding Buffer as well as X Solution at 4 °C to 30 °C
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