Insulin ELISA 试剂盒
(26 references)-
- 抗原 See all Insulin (INS) ELISA试剂盒
- Insulin (INS)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 nlU/mL - 1000 nlU/mL
- 最低检测浓度
- 15.6 nlU/mL
- 应用范围
- ELISA
- 原理
- For the quantitative determination of mouse insulin (INS) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of mouse INS. No significant cross-reactivity or interference between mouse INS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse INS and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 3.9 nlU/mL
- 组件
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- Assay plate
- Standard
- HRP-avidin (100 x concentrate)
- Biotin-antibody (100 x concentrate)
- Sample Diluent
- HRP-avidin Diluent
- Biotin-antibody Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Strip
- Top Product
- Discover our top product INS ELISA Kit
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Insulin (INS) ELISA Kit
INS 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 人 Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: Pig Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin (INS) ELISA KitINS 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin (INS) ELISA KitINS 适用: Cow Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 猴 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare reagents, samples and standards as instructed.
- Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
- Remove the liquid of each well, don't wash.
- Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
- Aspirate and wash 5 times.
- Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
- Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.
- 试剂准备
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- Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
- HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
- Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
- Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 1000 nIU/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (1000 nIU/mL). Sample Diluent serves as the zero standard (0 nIU/mL).
- Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
- Bring all reagents to room temperature (18-25 °C) before use for 30 min.
- Prepare fresh standard for each assay. Use within 4 hours and discard after use.
- Making serial dilution in the wells directly is not permitted.
- Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
- Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
- 样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
Note:Recommend to dilute the serum or plasma samples with Sample Diluent (1:200) before test. The suggested 200-fold dilution can be achieved by adding 5 μL sample to 45 μL of Sample Diluent. Complete the 200-fold dilution by adding15 μL of this solution to 285 μL of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments. - 实验精密度
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Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. - 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
- 有效期
- 6 months
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: "Berberine Improves Diabetic Encephalopathy Through the SIRT1/ER Stress Pathway in db/db Mice." in: Rejuvenation research, Vol. 21, Issue 3, pp. 200-209, (2018) (PubMed).
: "Kukoamine A attenuates insulin resistance and fatty liver through downregulation of Srebp-1c." in: Biomedicine & pharmacotherapy, Vol. 89, pp. 536-543, (2018) (PubMed).
: "Lycopene Improves Insulin Sensitivity through Inhibition of STAT3/Srebp-1c-Mediated Lipid Accumulation and Inflammation in Mice fed a High-Fat Diet." in: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, Vol. 125, Issue 9, pp. 610-617, (2018) (PubMed).
: "Arachidonic acid sex-dependently affects obesity through linking gut microbiota-driven inflammation to hypothalamus-adipose-liver axis." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1863, Issue 11, pp. 2715-2726, (2018) (PubMed).
: "Regenerative Potential of Spermatogonial Stem Cells, Endothelial Progenitor Cells, and Epithelial Progenitor Cells of C57Bl/6 Male Mice with Metabolic Disorders." in: Bulletin of experimental biology and medicine, Vol. 163, Issue 2, pp. 239-244, (2018) (PubMed).
: "6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 633-641, (2018) (PubMed).
: "Serine prevented high-fat diet-induced oxidative stress by activating AMPK and epigenetically modulating the expression of glutathione synthesis-related genes." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1864, Issue 2, pp. 488-498, (2018) (PubMed).
: "Role of Sertoli and Leydig Cells in the Regulation of Spermatogonial Stem Cell and Development of Reproductive Disorders in Male C57Bl/6 Mice with Type 1 Diabetes Mellitus." in: Bulletin of experimental biology and medicine, Vol. 164, Issue 2, pp. 127-131, (2018) (PubMed).
: "Targeted inhibition of RAGE reduces amyloid-β influx across the blood-brain barrier and improves cognitive deficits in db/db mice." in: Neuropharmacology, Vol. 131, pp. 143-153, (2018) (PubMed).
: "HIF1α deletion facilitates adipose stem cells to repair renal fibrosis in diabetic mice." in: In vitro cellular & developmental biology. Animal, Vol. 54, Issue 4, pp. 272-286, (2018) (PubMed).
: "Whole Body Vibration Improves Insulin Resistance in db/db Mice: Amelioration of Lipid Accumulation and Oxidative Stress." in: Applied biochemistry and biotechnology, Vol. 179, Issue 5, pp. 819-29, (2017) (PubMed).
: "Trivaric acid, a new inhibitor of PTP1b with potent beneficial effect on diabetes." in: Life sciences, Vol. 169, pp. 52-64, (2017) (PubMed).
: "Nuclear orphan receptor TLX affects gene expression, proliferation and cell apoptosis in beta cells." in: Biochemical and biophysical research communications, Vol. 468, Issue 1-2, pp. 387-93, (2016) (PubMed).
: "PPARγ agonists regulate bidirectional transport of amyloid-β across the blood-brain barrier and hippocampus plasticity in db/db mice." in: British journal of pharmacology, Vol. 173, Issue 2, pp. 372-85, (2016) (PubMed).
: "Dicaffeoylquinic Acid-Enriched Fraction of Cichorium glandulosum Seeds Attenuates Experimental Type 1 Diabetes via Multipathway Protection." in: Journal of agricultural and food chemistry, Vol. 63, Issue 50, pp. 10791-802, (2016) (PubMed).
: "Nr2e1 Deficiency Augments Palmitate-Induced Oxidative Stress in Beta Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 9648769, (2016) (PubMed).
: "WITHDRAWN: RIP140 mediates hyperglycemia-induced glucotoxicity in ?-cells via the activation of JNK and ERK1/2 signaling pathways." in: Diabetes research and clinical practice, (2015) (PubMed).
: "Homoarginine supplementation improves blood glucose in diet-induced obese mice." in: Amino acids, (2015) (PubMed).
: "Dan-Qi prescription ameliorates insulin resistance through overall corrective regulation of glucose and fat metabolism." in: Journal of ethnopharmacology, Vol. 172, pp. 70-9, (2015) (PubMed).
: "Antidiabetic drugs restore abnormal transport of amyloid-? across the blood-brain barrier and memory impairment in db/db mice." in: Neuropharmacology, Vol. 101, pp. 123-136, (2015) (PubMed).
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: "Berberine Improves Diabetic Encephalopathy Through the SIRT1/ER Stress Pathway in db/db Mice." in: Rejuvenation research, Vol. 21, Issue 3, pp. 200-209, (2018) (PubMed).
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- 抗原 See all Insulin (INS) ELISA试剂盒
- Insulin (INS)
- 别名
- Insulin,INS (INS 产品)
- 别名
- IDDM2 ELISA Kit, ILPR ELISA Kit, IRDN ELISA Kit, MODY10 ELISA Kit, ins1 ELISA Kit, xins ELISA Kit, ins1-a ELISA Kit, Insulin ELISA Kit, AA986540 ELISA Kit, Ins-2 ELISA Kit, InsII ELISA Kit, Mody ELISA Kit, Mody4 ELISA Kit, proinsulin ELISA Kit, zgc:109842 ELISA Kit, igf2-A ELISA Kit, ins ELISA Kit, ins-a ELISA Kit, ins-b ELISA Kit, insulin ELISA Kit, insulin precursor ELISA Kit, insulin II ELISA Kit, preproinsulin ELISA Kit, insulin L homeolog ELISA Kit, insulin S homeolog ELISA Kit, INS ELISA Kit, INS-IGF2 ELISA Kit, ins ELISA Kit, Ins ELISA Kit, PIN ELISA Kit, Ins2 ELISA Kit, ins.L ELISA Kit, ins.S ELISA Kit
- 背景
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Abbreviation: INS
Alias: N/A
- UniProt
- P01325
- 途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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