Hydroxyproline ELISA 试剂盒
(5 references)-
- 抗原 See all Hydroxyproline (Hyp) ELISA试剂盒
- Hydroxyproline (Hyp)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- For the quantitative determination of endogenic rat hydroxyproline (Hyp) concentrations in serum, plasma, tissue homogenates.
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of rat Hyp. No significant cross-reactivity or interference between rat Hyp and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat Hyp and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.078 ng/mL
- 组件
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- Assay plate
- Standard
- HRP-conjugate (100 x concentrate)
- Sample Diluent
- HRP-conjugate Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Strip
- Stop Solution
- Adhesive Strip
- Top Product
- Discover our top product Hyp ELISA Kit
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Hydroxyproline (Hyp) ELISA Kit
Hyp 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Hydroxyproline (Hyp) ELISA KitHyp 适用: Various Species Colorimetric Competition ELISA 61.7 ng/mL - 5000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hydroxyproline (Hyp) ELISA KitHyp 适用: 人 Colorimetric Sandwich ELISA 78 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
Hydroxyproline (Hyp) ELISA KitHyp 适用: 人 Colorimetric Sandwich ELISA 0.5-10 μmol/L Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Hydroxyproline (Hyp) ELISA KitHyp 适用: 大鼠 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 50 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare reagents, samples and standards as instructed.
- Set a Blank well without any solution.
- Add 50 µL standard or sample to each well.
- Add 50 µL HRP-conjugate (1x) to each well (Not to Blank well).
- Incubate 1 hour at 37 °C
- Aspirate and wash 5 times.
- Add 90 μL of TMB Substrate to each well. Incubate for 20 minutes at 37 °C. Protect from light.
- Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.
- 试剂准备
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- HRP-conjugate (1x) - Centrifuge the vial before opening. HRP-conjugate requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-conjugate + 990 μL of HRP-conjugate Diluent.
- Wash Buffer(1x)- If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
- Standard Centrifuge the standard vial at 6000-10000rpm for 30s before opening. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 10 ng/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 150 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (10 ng/mL).Sample Diluent serves as the zero standard (0 ng/mL).
- Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
- Bring all reagents to room temperature (18-25 °C) before use for 30 min.
- Prepare fresh standard for each assay. Use within 4 hours and discard after use.
- Making serial dilution in the wells directly is not permitted.
- Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
- Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
- 样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
Note:Serum and plasma samples require a 1000-fold dilution into Sample Diluent. The suggested 1000-fold dilution can be achieved by adding 5 μL sample to 95 μL of Sample Diluent first, then complete the 1000-fold dilution by adding 5 μL of this solution to 245 μL of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments. 6 - 实验精密度
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Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. - 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If don't make recent use, better keep it store at HRP-conjugate -20°C. Opened kit HRP-conjugate Diluent Sample Diluent May be stored for up to 1 month at 2 - 8°C. Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
- 有效期
- 6 months
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: "Abrogation of carbon tetrachloride-induced hepatotoxicity in Sprague-Dawley rats by Ajwa date fruit extract through ameliorating oxidative stress and apoptosis." in: Pakistan journal of pharmaceutical sciences, Vol. 30, Issue 6, pp. 2183-2191, (2019) (PubMed).
: "Effect of a new cross-linked hyaluronan gel on the staple line after sleeve gastrectomy in a rat model." in: Acta cirurgica brasileira, Vol. 33, Issue 2, pp. 163-174, (2018) (PubMed).
: "Protective Effects of Pterostilbene against Acetaminophen-Induced Hepatotoxicity in Rats." in: Journal of biochemical and molecular toxicology, Vol. 29, Issue 1, pp. 35-42, (2015) (PubMed).
: "Improving the angiogenic potential of collagen matrices by covalent incorporation of Astragalus polysaccharides." in: International journal of burns and trauma, Vol. 1, Issue 1, pp. 17-26, (2012) (PubMed).
: "Effects of long-term ethanol administration in a rat total enteral nutrition model of alcoholic liver disease." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 300, Issue 1, pp. G109-19, (2010) (PubMed).
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: "Abrogation of carbon tetrachloride-induced hepatotoxicity in Sprague-Dawley rats by Ajwa date fruit extract through ameliorating oxidative stress and apoptosis." in: Pakistan journal of pharmaceutical sciences, Vol. 30, Issue 6, pp. 2183-2191, (2019) (PubMed).
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- 抗原 See all Hydroxyproline (Hyp) ELISA试剂盒
- Hydroxyproline (Hyp)
- 别名
- hydroxyproline,Hyp (Hyp 产品)
- 物质类
- Amino Acid
- 背景
- Hyp
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