Osteopontin ELISA 试剂盒
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- 抗原 See all Osteopontin (SPP1) ELISA试剂盒
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.16 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.16 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes Mouse OPN in samples. No significant cross-reactivity or interference between Mouse OPN and analogues was observed.
- 灵敏度
- 0.1 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling SPP1 ELISA Kit
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- Discover our top product SPP1 ELISA Kit
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Secreted phosphoprotein 1 (SPP1) ELISA Kit
SPP1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA Milk, Plasma (EDTA), Tissue Culture Medium, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.3125 ng/mL - 10 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.06 ng/mL - 4 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 40 ng/mL Plasma, Serum, Urine
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- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18-25 °C) before use. If the kit will not be used up in one assay, please only take out the necessary strips and reagents for present experiment, and store the remaining strips and reagents at required condition.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 10 ng/mL(or add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 1-2 min and then mix it thoroughly with a vortex meter of low speed. Bubbles generated during vortex could be removed by centrifuging at a relatively low speed). Then make serial dilutions as needed. The recommended dilution gradient is as follows: 10, 5, 2.5, 1.25, 0.63, 0.32, 0.16, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500μL of Reference Standard & Sample Diluent to each tube. Pipette 500μL of the 10 ng/mL working solution to the first tube and mix up to produce a 5 ng/mL working solution. Pipette 500μL of the solution from the former tube into the latter one according to this step. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the Concentrated Biotinylated Detection Antibody at 800xg for 1 min, then dilute the 100x Concentrated Biotinylated Detection Antibody to 1x working solution with Biotinylated Detection Antibody Diluent(Concentrated Biotinylated Detection Antibody: Biotinylated Detection Antibody Diluent= 1: 99).
- HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the Concentrated HRP Conjugate at 800xg for 1 min, then dilute -6- the 100x Concentrated HRP Conjugate to 1x working solution with HRP Conjugate Diluent(Concentrated HRP Conjugate: HRP Conjugate Diluent= 1: 99). 10 5 2.5 1.25 0.63 0.32 0.16 0
- 样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse OPN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse OPN were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %. - 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- 有效期
- 12 months
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- 抗原 See all Osteopontin (SPP1) ELISA试剂盒
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- Osteopontin (SPP1 产品)
- 别名
- BNSP ELISA Kit, BSPI ELISA Kit, ETA-1 ELISA Kit, OPN ELISA Kit, 2AR ELISA Kit, Apl-1 ELISA Kit, Bsp ELISA Kit, Eta ELISA Kit, OP ELISA Kit, Opn ELISA Kit, Opnl ELISA Kit, Ric ELISA Kit, Spp-1 ELISA Kit, OSP ELISA Kit, zgc:111821 ELISA Kit, secreted phosphoprotein 1 ELISA Kit, SPP1 ELISA Kit, Spp1 ELISA Kit, spp1 ELISA Kit
- 背景
- SPP1, BNSP, BSP1, ETA-1, Bone Sialoprotein I, Secreted Phosphoprotein 1, Early T-Lymphocyte Activation 1
- 途径
- Regulation of Cell Size
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