CRP ELISA 试剂盒
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- 抗原 See all CRP ELISA试剂盒
- CRP (C-Reactive Protein (CRP))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.03 ng/mL - 25 ng/mL
- 最低检测浓度
- 0.03 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes natural and some recombinant Mouse CRP. No Significant cross- reactivity or interference between Mouse CRP and analogues was observed.
- 灵敏度
- 0.02 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling CRP ELISA Kit
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- Discover our top product CRP ELISA Kit
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C-Reactive Protein (CRP) ELISA Kit
High Sensitivity CRP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitCRP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitCRP 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitHigh Sensitivity CRP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鸡 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
C-Reactive Protein (CRP) ELISA KitCRP 适用: 豚鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Preheat the Microplate reader for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with deionized or distilled water to prepare 750 mL Wash Buffer. Note: if crystals have formed in the concentrate, warm it in 40 °C water bath and mix it gently until the crystals have completely dissolved.
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and turn it upside down for several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 25 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 25, 8.33, 2.78, 0.93, 0.31, 0.1, 0.03, 0 ng/mL. Dilution method: Take 7 EP tubes, add 600 μLof Reference Standard & Sample Diluent to each tube. Pipette 300 μLof the 25 ng/mL working solution to the first tube and mix up to produce a 8.33 ng/mL working solution. Pipette 300 μLof the solution from former tube to the latter one in order according to this step. The illustration below is for reference. Note: the last tube is regarded as blank. Don't pipette solution to it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before experiment (100 μL/well). In actual preparation, more account of 100~200μLshould be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before experiment (100 μL/well). In actual preparation, more account of 100~200 μL should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mouse CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mouse CRP were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %. - 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- 有效期
- 12 months
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- 抗原 See all CRP ELISA试剂盒
- CRP (C-Reactive Protein (CRP))
- 别名
- C-Reactive Protein (CRP 产品)
- 别名
- PTX1 ELISA Kit, crp ELISA Kit, AI255847 ELISA Kit, Aa1249 ELISA Kit, Ab1-341 ELISA Kit, Ab2-196 ELISA Kit, Ac1-114 ELISA Kit, Ac1262 ELISA Kit, Ac2-069 ELISA Kit, Ba2-693 ELISA Kit, APCS ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP2 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, ESP1 ELISA Kit, Hlp ELISA Kit, CRP5.1 ELISA Kit, zgc:152809 ELISA Kit, C-reactive protein ELISA Kit, C-reactive protein, pentraxin-related ELISA Kit, c-reactive protein, pentraxin-related ELISA Kit, cysteine rich protein 2 ELISA Kit, CRP ELISA Kit, crp ELISA Kit, Crp ELISA Kit, Crip2 ELISA Kit, LOC776376 ELISA Kit
- 途径
- Carbohydrate Homeostasis
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