SARS-CoV-2 N-Protein IgM Antibody ELISA 试剂盒
适用: 人, SARS Coronavirus-2 (SARS-CoV-2)
Colorimetric
Indirect ELISA
Plasma, Serum
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- 抗原
- SARS-CoV-2 N-Protein IgM Antibody
- 适用
- 人, SARS Coronavirus-2 (SARS-CoV-2)
- 检测方法
- Colorimetric
- 实验类型
- Indirect ELISA
- 应用范围
- ELISA
- 原理
- COVID-19 (SARS-CoV-2) N-Protein Human Antibody Detection Kit (Indirect ELISA method) for the semi-quantitative measurement of human IgM antibody against SARS-CoV-2 in serum or plasma.
- 样品类型
- Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 产品特性
- COVID-19 N-Protein Human IgM ELISA Kit
- 组件
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- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Stop Solution
- Assay Diluent
- Positive Control
- HRP-anti positive control IgG Antibody
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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SARS-CoV-2 N-Protein IgM Antibody ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 25 ng/mL - 200 ng/mL Plasma, Serum
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- 板类型
- Pre-coated
- 实验流程
- This COVID19 human IgM antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRPhuman IgM antibody-virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgM antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
- Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 12 months
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- 抗原
- SARS-CoV-2 N-Protein IgM Antibody
- 物质类
- Antibody
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