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SARS-CoV-2 N-Protein IgA Antibody ELISA 试剂盒

适用: 人, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Indirect ELISA Plasma, Serum
产品编号 ABIN6952758
发货至: 中国
  • 抗原
    SARS-CoV-2 N-Protein IgA Antibody
    适用
    人, SARS Coronavirus-2 (SARS-CoV-2)
    检测方法
    Colorimetric
    实验类型
    Indirect ELISA
    应用范围
    ELISA
    原理
    COVID-19 (SARS-CoV-2) N-Protein Human Antibody Detection Kit (Indirect ELISA method) for the semi-quantitative measurement of human IgA antibody against SARS-CoV-2 in serum or plasma.
    样品类型
    Plasma, Serum
    Analytical Method
    Semi-Quantitative
    产品特性
    COVID-19 N-Protein Human IgA ELISA Kit
    组件
    • SARS-CoV-2 N-protein coated 96-well microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent
    • Positive Control
    • HRP-anti positive control IgG Antibody
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 板类型
    Pre-coated
    实验流程
    This COVID19 human IgA antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRPhuman IgA antibody-virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    实验流程
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
    3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
    4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
    5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
    6. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    12 months
  • 抗原
    SARS-CoV-2 N-Protein IgA Antibody
    物质类
    Antibody
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