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ACE2 ELISA 试剂盒

ACE2 适用: 人 Colorimetric Sandwich ELISA 0.025 ng/mL - 20 ng/mL Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN6952471
发货至: 中国
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    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.025 ng/mL - 20 ng/mL
    最低检测浓度
    0.025 ng/mL
    应用范围
    ELISA
    原理
    Human ACE-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
    样品类型
    Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects Human ACE-2. Other species not determined.
    产品特性
    Human ACE-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water/li>
    • Precision pipettes to deliver 2 μL to 1 μL volumes/li>
    • Adjustable 1-25 μL pipettes for reagent preparation/li>
    • 100 μL and 1 liter graduated cylinders/li>
    • Tubes to prepare standard and sample dilutions/li>
    • Absorbent paper/li>
    • Microplate reader capable of measuring absorbance at 450nm/li>
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ACE2 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    样品制备

    Recommended Dilution (serum & plasma):8 fold

    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  • 抗原 See all ACE2 ELISA试剂盒
    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    别名
    ACE-2 (ACE2 产品)
    别名
    ACE1 ELISA Kit, CD143 ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit, ICH ELISA Kit, MVCD3 ELISA Kit, ACEH ELISA Kit, 2010305L05Rik ELISA Kit, zgc:92514 ELISA Kit, angiotensin I converting enzyme ELISA Kit, angiotensin I converting enzyme 2 ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 ELISA Kit, ACE ELISA Kit, ACE2 ELISA Kit, Ace2 ELISA Kit, ace2 ELISA Kit
    途径
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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