FH ELISA 试剂盒
FH
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
- 抗原 See all FH ELISA试剂盒
- FH (Fumarate Hydratase (FH))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Fumarate hydratase ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling FH ELISA Kit
- Top Product
- Discover our top product FH ELISA Kit
-
Fumarate Hydratase (FH) ELISA Kit
FH 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all FH ELISA试剂盒
- FH (Fumarate Hydratase (FH))
- 别名
- Fumarate hydratase (FH 产品)
- 别名
- FH ELISA Kit, DDBDRAFT_0205237 ELISA Kit, DDBDRAFT_0231397 ELISA Kit, DDBDRAFT_0231400 ELISA Kit, DDB_0205237 ELISA Kit, DDB_0231397 ELISA Kit, DDB_0231400 ELISA Kit, HLRCC ELISA Kit, LRCC ELISA Kit, MCL ELISA Kit, MCUL1 ELISA Kit, Fh1 ELISA Kit, im:7152785 ELISA Kit, ns:zf-e152 ELISA Kit, zf-e152 ELISA Kit, zgc:66253 ELISA Kit, zgc:77498 ELISA Kit, Fh ELISA Kit, Fh-1 ELISA Kit, fumarate hydratase ELISA Kit, fumarate hydratase 1 ELISA Kit, FH ELISA Kit, fumH ELISA Kit, Fh ELISA Kit, fh ELISA Kit, Fh1 ELISA Kit
- 基因ID
- 2271
- UniProt
- P07954
-
You are here:
