SMYD2A ELISA 试剂盒
SMYD2A
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SMYD2A products
- SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SMYD2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all SMYD2A products
- SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))
- 别名
- SMYD2 (SMYD2A 产品)
- 别名
- HSKM-B ELISA Kit, KMT3C ELISA Kit, ZMYND14 ELISA Kit, 1110020E07Rik ELISA Kit, 4930402C15 ELISA Kit, Zmynd14 ELISA Kit, smyd2-a ELISA Kit, im:7146196 ELISA Kit, smyd2 ELISA Kit, wu:fc02f02 ELISA Kit, zgc:110553 ELISA Kit, si:dkey-121j17.3 ELISA Kit, zgc:136609 ELISA Kit, SET and MYND domain containing 2 ELISA Kit, SET and MYND domain containing 2 S homeolog ELISA Kit, SET and MYND domain containing 2a ELISA Kit, SET and MYND domain containing 2b ELISA Kit, SMYD2 ELISA Kit, Smyd2 ELISA Kit, smyd2.S ELISA Kit, smyd2a ELISA Kit, smyd2b ELISA Kit
- 基因ID
- 56950
- UniProt
- Q9NRG4
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