Tyrosinase-Related Protein 1 ELISA 试剂盒
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- 抗原 See all Tyrosinase-Related Protein 1 (TYRP1) ELISA试剂盒
- Tyrosinase-Related Protein 1 (TYRP1)
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human TYRP1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TYRP1 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all Tyrosinase-Related Protein 1 (TYRP1) ELISA试剂盒
- Tyrosinase-Related Protein 1 (TYRP1)
- 别名
- TYRP1 (TYRP1 产品)
- 别名
- CAS2 ELISA Kit, CATB ELISA Kit, GP75 ELISA Kit, OCA3 ELISA Kit, TRP ELISA Kit, TRP1 ELISA Kit, TYRP ELISA Kit, b-PROTEIN ELISA Kit, LOC397853 ELISA Kit, Trypsin ELISA Kit, hm:zeh0659 ELISA Kit, tyrp1 ELISA Kit, zgc:100893 ELISA Kit, B ELISA Kit, TYRP1 ELISA Kit, tyrp-1 ELISA Kit, TRP-1 ELISA Kit, Tyrp ELISA Kit, b ELISA Kit, brown ELISA Kit, isa ELISA Kit, tyrosinase related protein 1 ELISA Kit, protease, serine 1 L homeolog ELISA Kit, tyrosinase-related protein 1b ELISA Kit, tyrosinase-related protein 1 ELISA Kit, tyrosinase-related protein 1 L homeolog ELISA Kit, tyrosinase-related protein 1a ELISA Kit, TYRP1 ELISA Kit, prss1.L ELISA Kit, tyrp1b ELISA Kit, Tyrp1 ELISA Kit, tyrp1.L ELISA Kit, tyrp1 ELISA Kit, LOC100136490 ELISA Kit, tyrp-1 ELISA Kit, tyrp1a ELISA Kit
- 基因ID
- 7306
- UniProt
- P17643
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