FHIT ELISA 试剂盒
FHIT
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all FHIT ELISA试剂盒
- FHIT (Fragile Histidine Triad (FHIT))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human FHIT ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling FHIT ELISA Kit
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- Discover our top product FHIT ELISA Kit
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Fragile Histidine Triad (FHIT) ELISA Kit
FHIT 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all FHIT ELISA试剂盒
- FHIT (Fragile Histidine Triad (FHIT))
- 别名
- FHIT (FHIT 产品)
- 别名
- AP3Aase ELISA Kit, FRA3B ELISA Kit, FHIT ELISA Kit, zgc:73176 ELISA Kit, AW045638 ELISA Kit, Fra14A2 ELISA Kit, LOC100223655 ELISA Kit, fragile histidine triad ELISA Kit, fragile histidine triad gene ELISA Kit, fragile histidine triad protein ELISA Kit, fragile histidine triad L homeolog ELISA Kit, FHIT ELISA Kit, Fhit ELISA Kit, fhit ELISA Kit, PY07476 ELISA Kit, fhit.L ELISA Kit
- 基因ID
- 2272
- UniProt
- P49789
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