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MZB1 ELISA 试剂盒
MZB1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
MZB1ELISA Kit详情
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MZB1
(Marginal Zone B and B1 Cell-Specific Protein (MZB1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Human MZB1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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有效期
6 months
MZB1目标详情
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MZB1
(Marginal Zone B and B1 Cell-Specific Protein (MZB1))
别名
MZB1 (MZB1 产品 )
别名
PACAP ELISA Kit, pERp1 ELISA Kit, MEDA-7 ELISA Kit, Pacap ELISA Kit, RGD1310251 ELISA Kit, 2010001M09Rik ELISA Kit, AV064572 ELISA Kit, marginal zone B and B1 cell specific protein ELISA Kit, marginal zone B and B1 cell-specific protein ELISA Kit, marginal zone B and B1 cell-specific protein 1 ELISA Kit, MZB1 ELISA Kit, Mzb1 ELISA Kit
基因ID
51237
UniProt
Q8WU39
途径
Production of Molecular Mediator of Immune Response
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