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ATG10 ELISA 试剂盒

ATG10 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951722
发货至: 中国
  • 抗原 See all ATG10 products
    ATG10 (Autophagy Related 10 (ATG10))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human ATG10 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all ATG10 products
    ATG10 (Autophagy Related 10 (ATG10))
    别名
    ATG10 (ATG10 产品)
    别名
    APG10 ELISA Kit, APG10L ELISA Kit, pp12616 ELISA Kit, Atg10 ELISA Kit, ATG10 ELISA Kit, MGC123341 ELISA Kit, im:7153729 ELISA Kit, zgc:123341 ELISA Kit, ATATG10 ELISA Kit, autophagy 10 ELISA Kit, DDBDRAFT_0190076 ELISA Kit, DDBDRAFT_0252608 ELISA Kit, DDB_0190076 ELISA Kit, DDB_0252608 ELISA Kit, 5330424L23Rik ELISA Kit, 5430428K15Rik ELISA Kit, AI852123 ELISA Kit, Agp10 ELISA Kit, Apg10l ELISA Kit, Apg10p ELISA Kit, Atg10l ELISA Kit, autophagy related 10 ELISA Kit, autophagy-related 10 ELISA Kit, AuTophaGy (yeast Atg homolog) ELISA Kit, ATG10 autophagy related 10 homolog (S. cerevisiae) ELISA Kit, autophagocytosis-associated family protein ELISA Kit, E2-like conjugating enzyme ELISA Kit, hypothetical protein ELISA Kit, autophagy protein 10 ELISA Kit, autophagy-related protein 10 ELISA Kit, ATG10 ELISA Kit, Atg10 ELISA Kit, atg-10 ELISA Kit, atg10 ELISA Kit, CAALFM_C302930WA ELISA Kit, APG10 ELISA Kit
    基因ID
    83734
    UniProt
    Q9H0Y0
    途径
    ER-Nucleus Signaling, Autophagy
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