RPRD1B ELISA 试剂盒
RPRD1B
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all RPRD1B products
- RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human RPRD1B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all RPRD1B products
- RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))
- 别名
- RPRD1B (RPRD1B 产品)
- 别名
- fb23h08 ELISA Kit, zgc:55687 ELISA Kit, wu:fb23h08 ELISA Kit, rprd1ba ELISA Kit, MGC130705 ELISA Kit, C13H20ORF77 ELISA Kit, C20orf77 ELISA Kit, CREPT ELISA Kit, NET60 ELISA Kit, dJ1057B20.2 ELISA Kit, 2610304G08Rik ELISA Kit, 2810446G03Rik ELISA Kit, Crept ELISA Kit, RGD1304782 ELISA Kit, regulation of nuclear pre-mRNA domain containing 1B ELISA Kit, regulation of nuclear pre-mRNA domain containing 1B L homeolog ELISA Kit, rprd1b ELISA Kit, rprd1b.L ELISA Kit, RPRD1B ELISA Kit, Rprd1b ELISA Kit
- 基因ID
- 58490
- UniProt
- Q9NQG5
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