GFPT1 ELISA 试剂盒
GFPT1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all GFPT1 ELISA试剂盒
- GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GFPT1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all GFPT1 ELISA试剂盒
- GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))
- 别名
- GFPT1 (GFPT1 产品)
- 别名
- gfat ELISA Kit, 2810423A18Rik ELISA Kit, AI324119 ELISA Kit, AI449986 ELISA Kit, GFA ELISA Kit, GFAT ELISA Kit, GFAT1 ELISA Kit, GFAT1m ELISA Kit, Gfpt ELISA Kit, CMSTA1 ELISA Kit, GFAT 1 ELISA Kit, GFPT ELISA Kit, GFPT1L ELISA Kit, MSLG ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 S homeolog ELISA Kit, glutamine fructose-6-phosphate transaminase 1 ELISA Kit, GFPT1 ELISA Kit, gfpt1.S ELISA Kit, Gfpt1 ELISA Kit
- 基因ID
- 2673
- UniProt
- Q06210
- 途径
- ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process
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