RUVBL1 ELISA 试剂盒
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- 抗原 See all RUVBL1 products
- RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human RUVBL1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling RUVBL1 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all RUVBL1 products
- RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))
- 别名
- RUVBL1 (RUVBL1 产品)
- 别名
- rvb1 ELISA Kit, ecp54 ELISA Kit, tip49 ELISA Kit, nmp238 ELISA Kit, pontin ELISA Kit, tip49a ELISA Kit, xpontin ELISA Kit, pontin52 ELISA Kit, TIP49 ELISA Kit, wu:fd18e04 ELISA Kit, zpontin ELISA Kit, ECP54 ELISA Kit, INO80H ELISA Kit, NMP238 ELISA Kit, PONTIN ELISA Kit, Pontin52 ELISA Kit, RVB1 ELISA Kit, TIH1 ELISA Kit, TIP49A ELISA Kit, Tip49a ELISA Kit, 2510009G06Rik ELISA Kit, RuvB like AAA ATPase 1 ELISA Kit, RuvB-like 1 (E. coli) ELISA Kit, RuvB-like 1 ELISA Kit, RuvB like AAA ATPase 1 S homeolog ELISA Kit, RuvB-like AAA ATPase 1 ELISA Kit, RuvB-like protein 1 ELISA Kit, RUVBL1 ELISA Kit, ruvbl1 ELISA Kit, PVX_123295 ELISA Kit, Bm1_15605 ELISA Kit, ruvb1 ELISA Kit, ruvbl1.S ELISA Kit, Ruvbl1 ELISA Kit
- 基因ID
- 8607
- UniProt
- Q9Y265
- 途径
- Telomere Maintenance
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