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PHGDH ELISA 试剂盒

PHGDH 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951683
发货至: 中国
  • 抗原 See all PHGDH ELISA试剂盒
    PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human PHGDH ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PHGDH ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all PHGDH ELISA试剂盒
    PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
    别名
    PHGDH (PHGDH 产品)
    别名
    fb38f06 ELISA Kit, zgc:65956 ELISA Kit, wu:fb38f06 ELISA Kit, embryo sac development arrest 9 ELISA Kit, F10M10.7 ELISA Kit, ECK2909 ELISA Kit, JW2880 ELISA Kit, 3-PGDH ELISA Kit, 3PGDH ELISA Kit, 4930479N23 ELISA Kit, A10 ELISA Kit, PGAD ELISA Kit, PGD ELISA Kit, PGDH ELISA Kit, SERA ELISA Kit, PDG ELISA Kit, phosphoglycerate dehydrogenase ELISA Kit, D-3-phosphoglycerate dehydrogenase ELISA Kit, 3-phosphoglycerate dehydrogenase ELISA Kit, phgdh ELISA Kit, EDA9 ELISA Kit, serA ELISA Kit, Sthe_2284 ELISA Kit, Dacet_1260 ELISA Kit, Mrub_0173 ELISA Kit, MMAH_RS07650 ELISA Kit, Arnit_0487 ELISA Kit, Ndas_0174 ELISA Kit, Mesil_2386 ELISA Kit, Slip_0010 ELISA Kit, Trad_0302 ELISA Kit, Acear_0027 ELISA Kit, Mahau_0665 ELISA Kit, MZHIL_RS05165 ELISA Kit, Mesop_1331 ELISA Kit, Thein_0855 ELISA Kit, Phgdh ELISA Kit, PHGDH ELISA Kit
    基因ID
    26227
    UniProt
    O43175
    途径
    Metabolism of Steroid Hormones and Vitamin D, Warburg Effect
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