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PSMA ELISA 试剂盒

FOLH1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951666
发货至: 中国
  • 抗原 See all PSMA (FOLH1) ELISA试剂盒
    PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human FOLH1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all PSMA (FOLH1) ELISA试剂盒
    PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
    别名
    FOLH1 (FOLH1 产品)
    别名
    FGCP ELISA Kit, FOLH ELISA Kit, GCP2 ELISA Kit, GCPII ELISA Kit, NAALAD1 ELISA Kit, NAALAdase ELISA Kit, PSM ELISA Kit, PSMA ELISA Kit, mGCP ELISA Kit, mopsm ELISA Kit, Naalad ELISA Kit, putative N-acetylated-alpha-linked acidic dipeptidase ELISA Kit, folate hydrolase 1B ELISA Kit, folate hydrolase 1 ELISA Kit, folate hydrolase (prostate-specific membrane antigen) 1 ELISA Kit, LOC451185 ELISA Kit, FOLH1B ELISA Kit, FOLH1 ELISA Kit, Folh1 ELISA Kit
    基因ID
    2346
    UniProt
    Q04609
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