SUB1 ELISA 试剂盒
SUB1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SUB1 ELISA试剂盒
- SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SUB1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SUB1 ELISA Kit
- Top Product
- Discover our top product SUB1 ELISA Kit
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Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1) ELISA Kit
SUB1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1) ELISA KitSUB1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all SUB1 ELISA试剂盒
- SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))
- 别名
- SUB1 (SUB1 产品)
- 别名
- P15 ELISA Kit, PC4 ELISA Kit, p14 ELISA Kit, AI842364 ELISA Kit, P9 ELISA Kit, Pc4 ELISA Kit, Rpo2tc1 ELISA Kit, SUB1 homolog, transcriptional regulator ELISA Kit, SUB1 homolog (S. cerevisiae) ELISA Kit, SUB1 ELISA Kit, Sub1 ELISA Kit
- 基因ID
- 10923
- UniProt
- P53999
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