CGREF1 ELISA 试剂盒
CGREF1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CGREF1 products
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CGREF1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all CGREF1 products
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
- 别名
- CGREF1 (CGREF1 产品)
- 别名
- CGR11 ELISA Kit, CGREF1 ELISA Kit, 1110004G24Rik ELISA Kit, AW045201 ELISA Kit, Cgr11 ELISA Kit, cell growth regulator with EF-hand domain 1 ELISA Kit, cell growth regulator with EF-hand domain 1 L homeolog ELISA Kit, cell growth regulator with ring finger domain 1 ELISA Kit, cell growth regulator with EF hand domain 1 ELISA Kit, CGREF1 ELISA Kit, cgref1.L ELISA Kit, cgref1 ELISA Kit, CGRRF1 ELISA Kit, Cgref1 ELISA Kit
- 基因ID
- 10669
- UniProt
- Q99674
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