CES5A ELISA 试剂盒
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- 抗原 See all CES5A ELISA试剂盒
- CES5A (Carboxylesterase 5A (CES5A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse CES5A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Carboxylesterase 5A (CES5A) ELISA Kit
CES5A 适用: 大鼠 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Carboxylesterase 5A (CES5A) ELISA KitCES5A 适用: 小鼠 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Carboxylesterase 5A (CES5A) ELISA KitCES5A 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all CES5A ELISA试剂盒
- CES5A (Carboxylesterase 5A (CES5A))
- 别名
- CES5A (CES5A 产品)
- 别名
- CAUXIN ELISA Kit, CES4C1 ELISA Kit, CES5 ELISA Kit, CES7 ELISA Kit, 1700081L16Rik ELISA Kit, 1700122C07Rik ELISA Kit, BB081581 ELISA Kit, Ces7 ELISA Kit, Gm503 ELISA Kit, cauxin ELISA Kit, Ces5 ELISA Kit, LOC445455 ELISA Kit, carboxylesterase 5A ELISA Kit, CES5A ELISA Kit, Ces5a ELISA Kit
- 基因ID
- 67935
- UniProt
- Q6AW46
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