HAAO ELISA 试剂盒
HAAO
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all HAAO ELISA试剂盒
- HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat HAAO ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling HAAO ELISA Kit
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- Discover our top product HAAO ELISA Kit
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3-hydroxyanthranilate 3,4-Dioxygenase (HAAO) ELISA Kit
HAAO 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all HAAO ELISA试剂盒
- HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))
- 别名
- HAAO (HAAO 产品)
- 别名
- Afu2g17450 ELISA Kit, 0610007K21Rik ELISA Kit, 0610012J07Rik ELISA Kit, 3-HAO ELISA Kit, 3-HAOxase ELISA Kit, 3HAO ELISA Kit, zgc:103585 ELISA Kit, HAO ELISA Kit, 3-hydroxyanthranilate 3,4-dioxygenase ELISA Kit, 3-hydroxyanthranilate 3,4-dioxygenase L homeolog ELISA Kit, SPO1774 ELISA Kit, CNB05620 ELISA Kit, AFUA_2G17450 ELISA Kit, Bcep18194_C7641 ELISA Kit, Kfla_5491 ELISA Kit, Micau_4935 ELISA Kit, RBRH_03005 ELISA Kit, Krodi_1278 ELISA Kit, haao ELISA Kit, HAAO ELISA Kit, Haao ELISA Kit, haao.L ELISA Kit
- 基因ID
- 56823
- UniProt
- P46953
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