PVRL1 ELISA 试剂盒
PVRL1
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all PVRL1 ELISA试剂盒
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PVRL1
(Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
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适用
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大鼠
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Rat PVRL1/Nectin-1 ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注
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Optimal working dilution should be determined by the investigator.
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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有效期
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6 months
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抗原
See all PVRL1 ELISA试剂盒
-
PVRL1
(Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
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别名
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PVRL1 (Nectin-1 (PVRL1 产品)
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别名
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CD111 ELISA Kit, CLPED1 ELISA Kit, ED4 ELISA Kit, HIgR ELISA Kit, HVEC ELISA Kit, OFC7 ELISA Kit, PRR ELISA Kit, PRR1 ELISA Kit, PVRR ELISA Kit, PVRR1 ELISA Kit, SK-12 ELISA Kit, nectin-1 ELISA Kit, prr1 ELISA Kit, PVRL1 ELISA Kit, pvrl1 ELISA Kit, AI835281 ELISA Kit, AW549174 ELISA Kit, Cd111 ELISA Kit, HveC ELISA Kit, cd111 ELISA Kit, cd112 ELISA Kit, clped1 ELISA Kit, ed4 ELISA Kit, higr ELISA Kit, hveb ELISA Kit, hvec ELISA Kit, nectin-2 ELISA Kit, ofc7 ELISA Kit, prr ELISA Kit, prr2 ELISA Kit, pvrl2 ELISA Kit, pvrl2.L ELISA Kit, pvrr ELISA Kit, pvrr1 ELISA Kit, pvrr2 ELISA Kit, sk-12 ELISA Kit, nectin cell adhesion molecule 1 ELISA Kit, nectin-1 ELISA Kit, Poliovirus receptor-related protein 1 ELISA Kit, poliovirus receptor-related 1 (herpesvirus entry mediator C) ELISA Kit, nectin cell adhesion molecule 2 L homeolog ELISA Kit, NECTIN1 ELISA Kit, LOC100145553 ELISA Kit, pvrl1 ELISA Kit, nectin1 ELISA Kit, Nectin1 ELISA Kit, PVRL1 ELISA Kit, nectin2.L ELISA Kit
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基因ID
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192183
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UniProt
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F1LNP8
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途径
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Cell-Cell Junction Organization
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