ATG3 ELISA 试剂盒
ATG3
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ATG3 ELISA试剂盒
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ATG3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ATG3 ELISA Kit
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ATG3 Autophagy Related 3 (ATG3) ELISA Kit
ATG3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ATG3 Autophagy Related 3 (ATG3) ELISA KitATG3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all ATG3 ELISA试剂盒
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- 别名
- ATG3 (ATG3 产品)
- 别名
- atg3 ELISA Kit, im:6910145 ELISA Kit, wu:fb15g12 ELISA Kit, zgc:64156 ELISA Kit, APG3 ELISA Kit, APG3-LIKE ELISA Kit, APG3L ELISA Kit, PC3-96 ELISA Kit, 2610016C12Rik ELISA Kit, Apg3l ELISA Kit, Atg3l ELISA Kit, PIG-1 ELISA Kit, Pig1 ELISA Kit, ATATG3 ELISA Kit, K11J9.1 ELISA Kit, K11J9_1 ELISA Kit, autophagy related 3 ELISA Kit, autophagy 3 (APG3) ELISA Kit, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy ELISA Kit, atg3 ELISA Kit, ATG3 ELISA Kit, Atg3 ELISA Kit
- 基因ID
- 64422
- UniProt
- Q9NT62
- 途径
- Autophagy
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