PHAP1 ELISA 试剂盒
ANP32A
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PHAP1 (ANP32A) ELISA试剂盒
- PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ANP32A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all PHAP1 (ANP32A) ELISA试剂盒
- PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))
- 别名
- ANP32A (ANP32A 产品)
- 别名
- C15orf1 ELISA Kit, HPPCn ELISA Kit, I1PP2A ELISA Kit, LANP ELISA Kit, MAPM ELISA Kit, PHAP1 ELISA Kit, PHAPI ELISA Kit, PP32 ELISA Kit, Anp32 ELISA Kit, W91701 ELISA Kit, pp32 ELISA Kit, Anp32a ELISA Kit, CG5784 ELISA Kit, CT18148 ELISA Kit, CT42180 ELISA Kit, Dmel\\CG5784 ELISA Kit, anon-EST:fe3A2 ELISA Kit, wu:fj36e08 ELISA Kit, zgc:55516 ELISA Kit, ANP32D ELISA Kit, acidic nuclear phosphoprotein 32 family member A ELISA Kit, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A ELISA Kit, CG5784 gene product from transcript CG5784-RB ELISA Kit, acidic nuclear phosphoprotein 32 family member A L homeolog ELISA Kit, ANP32A ELISA Kit, Anp32a ELISA Kit, Mapmodulin ELISA Kit, anp32a ELISA Kit, anp32a.L ELISA Kit
- 基因ID
- 8125
- UniProt
- P39687
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