14-3-3 theta ELISA 试剂盒
-
- 抗原 See all 14-3-3 theta (YWHAQ) ELISA试剂盒
- 14-3-3 theta (YWHAQ)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human 14-3-3 theta ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling YWHAQ ELISA Kit
- Top Product
- Discover our top product YWHAQ ELISA Kit
-
14-3-3 theta (YWHAQ) ELISA Kit
YWHAQ 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
14-3-3 theta (YWHAQ) ELISA KitYWHAQ 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all 14-3-3 theta (YWHAQ) ELISA试剂盒
- 14-3-3 theta (YWHAQ)
- 别名
- 14-3-3 theta (YWHAQ 产品)
- 别名
- 14-3-3 ELISA Kit, 14-3-3t ELISA Kit, 1c5 ELISA Kit, hs1 ELISA Kit, 1C5 ELISA Kit, HS1 ELISA Kit, 2700028P07Rik ELISA Kit, AA409740 ELISA Kit, AU021156 ELISA Kit, R74690 ELISA Kit, ywhaq ELISA Kit, ywhaq2 ELISA Kit, wu:fa91b01 ELISA Kit, wu:fb02e12 ELISA Kit, wu:fb36f06 ELISA Kit, wu:fk86h02 ELISA Kit, ywhaq1 ELISA Kit, ywhaz ELISA Kit, zgc:55499 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a ELISA Kit, 14-3-3 protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b ELISA Kit, YWHAQ ELISA Kit, ywhaq ELISA Kit, Ywhaq ELISA Kit, ywhaq.S ELISA Kit, ywhaqa ELISA Kit, ywhaqb ELISA Kit
- 基因ID
- 10971
- UniProt
- P27348
- 途径
- Apoptosis, Myometrial Relaxation and Contraction
-
