14-3-3 sigma/SFN ELISA 试剂盒
-
- 抗原 See all 14-3-3 sigma/SFN (SFN) ELISA试剂盒
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse SFN ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SFN ELISA Kit
- Top Product
- Discover our top product SFN ELISA Kit
-
Stratifin (SFN) ELISA Kit
SFN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Stratifin (SFN) ELISA KitSFN 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all 14-3-3 sigma/SFN (SFN) ELISA试剂盒
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- 别名
- SFN (SFN 产品)
- 别名
- YWHAS ELISA Kit, Er ELISA Kit, Mme1 ELISA Kit, Ywhas ELISA Kit, stratifin ELISA Kit, SFN ELISA Kit, Sfn ELISA Kit
- 基因ID
- 55948
- UniProt
- O70456
- 途径
- p53 Pathway, Myometrial Relaxation and Contraction
-
