Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA 试剂盒
CMPK1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA试剂盒
- Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CMPK1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA试剂盒
- Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
- 别名
- CMPK1 (CMPK1 产品)
- 别名
- CK ELISA Kit, CMK ELISA Kit, CMPK ELISA Kit, UMK ELISA Kit, UMP-CMPK ELISA Kit, UMPK ELISA Kit, UMP/CMPK ELISA Kit, PSPTO1749 ELISA Kit, cmpk1 ELISA Kit, fb52d08 ELISA Kit, fk47a07 ELISA Kit, ump ELISA Kit, wu:fb52d08 ELISA Kit, wu:fk47a07 ELISA Kit, zgc:56452 ELISA Kit, Cmpk ELISA Kit, RGD1310116 ELISA Kit, cmpk ELISA Kit, 0610011D08Rik ELISA Kit, UMP-CMPk ELISA Kit, UCK ELISA Kit, cytidine/uridine monophosphate kinase 1 ELISA Kit, cytidylate kinase ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic S homeolog ELISA Kit, cytidylate kinase Cmk ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1 ELISA Kit, CMPK1 ELISA Kit, cmk ELISA Kit, cmpk1 ELISA Kit, cmpk ELISA Kit, Cmpk1 ELISA Kit, cmpk1.S ELISA Kit, Mrub_0478 ELISA Kit, MMAH_RS06670 ELISA Kit, TAGG_RS04320 ELISA Kit, Trad_0784 ELISA Kit, Deba_2713 ELISA Kit, Toce_1154 ELISA Kit, VDIS_RS03585 ELISA Kit, Fbal_1939 ELISA Kit, Ilyop_1379 ELISA Kit, Lbys_3481 ELISA Kit, Calni_0093 ELISA Kit, Ftrac_1556 ELISA Kit, Ocepr_1777 ELISA Kit
- 基因ID
- 51727
- UniProt
- P30085
- 途径
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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