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UBE2D1 ELISA 试剂盒

UBE2D1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951479
发货至: 中国
  • 抗原 See all UBE2D1 products
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human UBE2D1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all UBE2D1 products
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    别名
    UBE2D1 (UBE2D1 产品)
    别名
    E2(17)KB1 ELISA Kit, SFT ELISA Kit, UBC4/5 ELISA Kit, UBCH5 ELISA Kit, UBCH5A ELISA Kit, ube2d1 ELISA Kit, wu:fc16h06 ELISA Kit, zgc:73096 ELISA Kit, e2(17)kb1 ELISA Kit, sft ELISA Kit, ubc4/5 ELISA Kit, ubch5 ELISA Kit, ubch5a ELISA Kit, ubiquitin conjugating enzyme E2 D1 ELISA Kit, ubiquitin-conjugating enzyme E2D 1 ELISA Kit, ubiquitin-conjugating enzyme E2D 1b ELISA Kit, ubiquitin conjugating enzyme E2 D1 S homeolog ELISA Kit, UBE2D1 ELISA Kit, Ube2d1 ELISA Kit, ube2d1b ELISA Kit, ube2d1.S ELISA Kit, ube2d1 ELISA Kit
    基因ID
    7321
    UniProt
    P51668
    途径
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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