IGFBP4 ELISA 试剂盒
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- 抗原 See all IGFBP4 ELISA试剂盒
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse IGFBP-4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product IGFBP4 ELISA Kit
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Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit
IGFBP4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 小鸡 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: Cow Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 人 AA 22-258 Colorimetric Sandwich ELISA 500-32000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 人 Colorimetric Sandwich ELISA 500 pg/mL - 32000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 山羊 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all IGFBP4 ELISA试剂盒
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- 别名
- IGFBP-4 (IGFBP4 产品)
- 别名
- IGFBP4 ELISA Kit, igfbp4 ELISA Kit, BP-4 ELISA Kit, HT29-IGFBP ELISA Kit, IBP4 ELISA Kit, IGFBP-4 ELISA Kit, AI875747 ELISA Kit, Deb2 ELISA Kit, IGF-BP4 ELISA Kit, IBP-4 ELISA Kit, insulin like growth factor binding protein 4 ELISA Kit, insulin-like growth factor-binding protein 4 ELISA Kit, insulin-like growth factor binding protein 4 ELISA Kit, IGFBP4 ELISA Kit, LOC100136305 ELISA Kit, LOC100194498 ELISA Kit, igfbp4 ELISA Kit, Igfbp4 ELISA Kit
- 基因ID
- 16010
- UniProt
- P47879
- 途径
- WNT signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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